Effect of bone morphogenetic protein-2 combined with hyperthermic chemotherapy on GDF15 and TFF3 expression in SW480

Abstract:

Objective：To explore the effect of bone morphogenetic protein-2（BMP-2） combined with hyperthermic chemotherapy on GDF15 and TFF3 expression in SW480. Methods：BMP-2 was acted on SW480 colorectal cancer cell lines，placed in 43 ℃ hyper－thermic chemotherapy for 30 min and cultured for 6 h. （1）Apoptosis of SW480 cells was detected by flow cytometry assay；（2）GDF15 and TFF3 protein expressions were detected by Western blot；（3）GDF15 and TFF3 mRNA expressions were detected by RT-PCR. Results：SW480 cells apoptosis was increased，GDF15 and TFF3 protein levels were decreased after BMP-2 combined with 43 ℃ hyperthermic chemotherapy. GDF15 and TFF3 mRNA expressions were also reduced. Conclusions：BMP-2 combined hyperthermic chemotherapy can inhibit proliferation and metastasis of SW480 by inhibiting GDF15 and TFF3 protein and mRNA expressions. Hyperthermia combined with chemotherapy has synergistic effect on the inhibition of GDF15 and TFF3 expression.