Construction of subtracted cDNA library of diferentially expressed genes of multidrug resistant tubercle bacillus by suppression subtracted hybridization

Abstract:

Objective：To build the subtracted cDNA library of differentially expressed genes of multidrug-resistant tubercle bacillus（MDR-TB）and to further discuss the molecular mechanism of MDR-TB. Methods：Tester was MDR-TB and Driver was sensitive tu－berculosis. Suppression subtractive hybridization（SSH） and T/A cloning technology were done to build the subtracted cDNA library of differentially expressed genes of MDR-TB. Results：The subtracted cDNA library of differentially expressed genes of MDR-TB was successfully built and 113 differentially expressed cDNA fragements of MDR-TB were obtained. Sequencing and homology analysis showed that 5 of them were novel cDNA sequences and 5 sequenced genes were reported to be related with MDR in TB. Conclusions：SSH is an effective method for screening new function genes. Many genes both known and unknown are in correlation with MDR in TB. Discovery of these genes provides a solid foundation for the explanation of MDR mechanism in TB.