Effect of Puerarin on the proliferation of choroidal vascular endothelial cells in vitro

Abstract:

Objective：To investigate the effect of different concentrations of Puerarin（Pur） on the proliferation of choroidal vascular endothelial cells（CVECs） in vitro. Methods：The third generation of CVECs were used for the research. The CVECs in experimental group were exposed to different concentrations of Pur while those in cell control group were exposed to equal amount of blank medi－um. The effect of Pur on the proliferation of cells was measured by methyl thiazolyl thiazolium（MTT） assay. Results：CVECs cultured with 0.05，0.50，5.00 μg/ml and 50.00 μg/ml Pur showed increase in absorbance（A） value at 24 h and 48 h after treatment（P<0.01）. Conclusion：Pur can obvious promote the proliferation of CVECs，which is related with the dosage of Pur.