Genotype of human papilloma virus infection in Xinjiang

Abstract:

Objective：To investigate the genotypes of human papilloma viruses（HPV）in women with different gynecologic diseases in Xinjiang by PCR-reverse dot blot（PCR-RDB）. Methods：Clinical specimens from 335 women with suspected symptoms of gynecologic disorders were collected and HPV DNA（L1） was isolated and subjected to genotyping by PCR-RDB. HPV infection rates were cal－culated and statistical analysis of χ2 test was performed to compare the HPV infection rates among different groups. Results：Totally 108 specimens were identified to be HPV positive and the posotive rate was 32.2%（108/335）. Nine kinds of HPV genotypes（HPV 16，18，33，45，56，68，6，11，66） were identified by PCR-RDB and HPV 16，18，56 had higher infection rates than other HPV genotypes. Statistic analysis showed that patients with condyloma，cervical intraepithelial neoplasiaⅡ/Ⅲ and cervical cancer had significantly higher HPV positive rates and high-risk HPV infection rates than those with cervicitis or cervical erosion（P＜0.05）. Additionally，HPV infection rates were decreased with the increasing of age. Conclusions：HPV infection is common in patients with different uter－ine-cervical diseases in Xinjiang and HPV genotyping by PCR-RDB could provide epidemiological evidence for the diagnosis，treat－ment and follow-up of HPV infection.