Effects of exercise training on expressions of LINGO-1 in the brain of rats and neural function after focal cerebral infarction

Abstract:

Objective：To observe the expressions of LINGO-1 in the brain of rats after exercise training and to explore the possible molecular mechanism of exercise training promoting neurological functional recovery after stroke. Methods：Middle cerebral artery oc－clusion（MCAO） model was made and rats were randomly divided into sham operation group，model group and exercise training group. Observation was made at 3，7，14 d after MCAO. Training method was treadmill exercise. Neurological functional recovery was evalu－ated by Longa’s score；expressions of LINGO-1 mRNA and protein were detected by imunohistochemistry and RT-PCR. Results：Sham group had no obvious neurological deficiency（Longa score was 0） but small amount of mRNA and protein expressions of LIN－GO-1. mRNA and protein expressions of LINGO-1 in model group were reached the peak at 3 d after MCAO and decreased gradual－ly later（P<0.05）. There was no difference in mRNA and protein expressions of LINGO-1 between exercise training group and model group at 3，7 d after MCAO（P >0.05）. Longa score and mRNA and protein expressions of LINGO-1 were decreased at 14 d after MCAO in exercise training group compared with those in model group（P<0.05）. Conclusions：Expressions of LINGO-1 in the is－chemic brain are increased significantly. Decreased neurological score and LINGO-1 mRNA and protein expressions induced by prop－er exercise training might be one of the molecular mechanisms of neural function recovery after acute cerebral infarction.