Protein aiginine methyltransferase 4 and methylated metabolite asymmetric dimethylarginines in polycystic ovary syndrome

Abstract:

Objective：To investigate levels of asymmetric dimethylarginines（ADMA） in serum of women with and without polycystic ovary syndrome（PCOS），PCOS model rats and control rats，to explore expressions of protein aiginine methyltransferase 4（PRMT4） in ovarian tissues of PCOS model rats and to explore relationship between levels of ADMA and PRMT4 and PCOS. Methods：ELISA was used to investigate levels of ADMA in serum of 49 PCOS patients，20 normal patients，20 PCOS model rats and 20 normal rats. Ex－pressions of PRMT4 in ovarian tissues were detected by SP immunohistochemical method（including 20 PCOS model rats and 20 con－trol rats）. Results：（１）In human serum，ADMA concentrations were （0.49±0.35）umol/L and （0.26±0.09） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （２）In rat serum，ADMA concentrations were （0.55±0.14） umol/L and （0.23±0.08） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. （３）In rat ovarian tissues，PRMT4 integral optical density values were （44.96±9.40） umol/L and （18.27±3.83） umol/L in PCOS group and control group respectively，with significant differences between two groups（P=0.00）. Conclusions：In ovar－ian tissues，increased expressions of PRMT4 may bring about elevated ADMA level in serum，which causes endothelial dysfunction of PCOS and affects PCOS development.