Research on extracting DNA of human tissues after high-temperature disposal

Abstract:

Objective：To study the effects of DNA extraction methods on deoxyribonucleic acid-short tandem repeats（DNA-STR） typ－ing of human tissue after high-temperature disposal. Methods：Human tissue samples after high-temperature disposal were initially handled with different concentrations of TritonX-100. DNA was extracted using the method of Chelex-100 and was amplified by re－combination of DNA-STR. Then，ABI-3130 sequence analyzer was used for electrophoresis separation and Gene mapper ID V3.2 gene analysis software was used for gene typing. Results：1% TritonＸ-100+Chelex-100 was used to process the samples before DNA ex－traction. All loci could be detected after QIA quick PCR purification and the loci could be balancedly amplified. Conclusions：This is a simple method with satisfied results，which is recommended in the field of forensic science，especially for the prosecution case.