Anti-tumor effects of dendritic cell vaccine carrying HPV-16 E6/E7 gene on the transplantable tumor of human cervical cancer in nude mice

Abstract:

Objective：To explore the anti-tumor effects of dendritic cell（DC） vaccine carrying HPV-16 E6/E7 gene on the CaSki cell transplantable tumor of human cervical cancer in nude mice. Methods：Immature C57BL/6 DC（imDC） was cultured and was infected with recombinant adenovirus vector carrying human papillomavirus-16 E6/E7 gene. Expressions of E7 protein were detected by West－ern blot. CaSki cell transplantable tumor model of human cervical cancer was constructed in nude mice and was treated by cytotoxic T lymphocytes（CTL），acquired from BALB/c mice. Changes in tumor volume were observed. Nude mice were sacrificed and Paraffin-embedded on the 28th d since tumors appeared. Tumor cell morphology was observed in each group by HE staining. Expressions of Bcl-2 and Bax protein of tumor tissues were detected by immunohistochemical techniques. Results：DC was successfully cultured and DC vaccine was prepared. On the 28th d，tumor volume of nude mice was smaller in pAd-E6/E7-DC-CTL-CaSki group than in pAd-mock-DC-CTL-CaSki group，DC-CTL-CaSki group and CaSki group（P<0.05）. Bax protein expressions of tumor tissues detected by immunohistochemical techniques demonstrated that：expressions in pAd-E6/E7-DC-CTL-CaSki group were the highest while in CaS－ki group were the lowest. Bcl-2 protein expressions of tumor tissue detected by immunohistochemical techniques demonstrated that：expressions in pAd-E6/E7-DC-CTL-CaSki group were the lowest while those in CaSki group were the highest. Conclusions：DC vac－cine modified by HPV-16 E6/E7 gene can inhibit the grow of cervical cancer transplantable tumor in nude mice by inducing CTL.