Role of Caspase-12 expression and cytochrome-C release in oligodendrocyte apoptosis induced by compressed spinal cord injury

Abstract:

Objective：To evaluate the role of Caspase-12 expression and cytochrome-C（Cyto-C） release in oligodendrocyte apoptosis induced by compressed spinal cord injury（CSCI）. Methods：CSCI model was established with a self-made device. Oligodendrocyte apoptosis was determined by TdT mediated dUTP-biotin nick end labeling staining at 1，3，7 d after compression. Cyto-C release and Caspase-12 expressions were detected by Western blot. Results：Numbers of oligodendrocyte apoptosis in different groups were differ－ent（F=30.946，P=0.000）. Numbers of oligodendrocyte apoptosis in 1，3，7 d groups were more than those in control group according to LSD-t（P=0.000）. Numbers of oligodendrocyte apoptosis in 7 d group reached the maximum compared with those in control group，1 d group and 3 d group，with statistically significant differences among groups（P=0.000）. Both expressions of Caspase-12 and releases of Cyto-C in different groups were different（F=1 402.646 and 326.638 respectively，P=0.000）. Expressions of Caspase-12 and releas－es of Cyto-C in 1，3，7 d groups were more than those in control group according to LSD-t（P=0.000）. Expressions of Caspase-12 and releases of Cyto-C in 7 d group reached the maximum compared with those in control group，1 d group and 3 d group，with statistically significant differences among groups（P=0.000）. Conclusions：Oligodendrocyte apoptosis after CSCI is associated with enhanced expressions of Caspase-12 and releases of Cyto-C.