Effects of Yangxueqingnao granule on inflammatory factor expressions and axonal regeneration in rats with focal cerebral ischemia reperfusion

Abstract:

Objective：To investigate the effects of Yangxueqingnao granule on expressions of nuclear factor-κBp65（NF-κBp65），in－terleukin-6（IL-6） and neurofilament protein-200（NF-200） in cortex and neurological function after focal cerebral ischemia reperfu－sion injury in rats. Methods:Seventy-two SD rats were randomly divided into sham-operated group（S group），cerebral ischemia/reperfusion group（I/R group），normal saline control group（I/R+C group） and Yangxueqingnao granule treated group（I/R+YXQ group）. Neurological function was measured using Montoya staircase test. Changes in expressions of NF-κBp65 and IL-6 were analyzed by immunohistochemistry and Western blot，respectively. NF-200 immunohistochemical staining was used to evaluate axonal regener－ation. Results：At the 2nd d after ischemia reperfusion，expressions of NF-κBp65 and IL-6 in ischemic cortex were significantly in－creased（P<0.001），while those of NF-200 were significantly decreased in I/R group（P<0.001）. Expressions of NF-κBp65 and IL-6 were lower in IR+YXQ group than in I/R group（P<0.001）. At the 2nd week after ischemia reperfusion，expressions of NF-200 positive cells in I/R+YXQ group were increased compared with those in the I/R group（P<0.001） and behavioral tests showed no difference in neurological improvement（P=0.178）. Conclusions：Yangxueqingnao granule can improve neurological function recovery after focal cerebral ischemic reperfusion injury in rats，which may be associated with the inhibition of inflammation reactions.