Effects of over-expressed SorLA on β-amyloid in Alzheimer’s disease

Abstract:

Objective：To investigate effects of over-expressed SorLA on β-amyloid（Aβ） in Alzheimer’s disease and to reveal the relation between SorLA expressions and the total amount of Aβ. Methods：Western blot was used to analyze the levels of SorLA protein in brain tissue. Human embryonic kidney cells（HEK-293） were transfected with the plasmids carrying the SorLA interfered by SorLA cDNA（screened by G418） and expression levels of amyloid precursor protein（APP） were analyzed by Western blot. Levels of Aβ were measured by ELISA. Results：（1）Western blot showed that levels of APP were significantly reduced in the over-expression Sor－LA transfected group than in control group. （2）ELISA showed that the total amount of Aβ was reduced by（21.6±12.1）％ obviously（P<0.01）. Conclusions：Total amount of Aβ is directly affected by levels of SorLA protein and they are negatively correlated.