Regulation of interleukin-27 in brain antigen-presenting cells by central nervous system inflammation

Abstract:

Objective：To explore how inflammatory factors and apoptotic cells regulate central nervous system（CNS） antigen-present－ing cells（APCs） related cytokine-interleukin-27（IL-27） expression in inflammation. Methods：qRT-PCR was used to measure and compare IL-27 p28 mRNA relative expression in mice microglia cells and bone marrow derived dendritic cells（BMDCs），treated with lipopolysaccharide（LPS），interferon-γ（IFN-γ） and X-ray induced apoptotic cells. Western blot was performed to detect IL-27 ex－pression in different stimuli groups. Results：Expressions of IL-27 p28 mRNA and IL-27 were up-regulated when microglia cells and BMDCs being treated with LPS and increased expressions of IL-27 p28 and IL-27 were in relation with LPS dosages. Expressions of IL-27 p28 mRNA and IL-27 were significantly up-regulated when microglia cells and BMDCs being treated with IFN-γ plus LPS（microglia cells：PLPS 0.50 μg/ml=0.029，PLPS+IFN-γ=4×10-7；BMDCs：P0.50 μg/ml=0.021，PLPS+IFN-γ=8×10-7）. Expressions of IL-27 p28 mRNA and IL-27 were significantly down-regulated when microglia cells and BMDCs being pre-treated with apoptotic cells（microglia cells：PLPS+apoptotic cells=1.2×10-4，PIFN-γ+LPS+apoptotic cells=1×10-7；BMDCs：PLPS+apoptotic cells=3×10-4，PIFN-γ+LPS+apoptotic cells=2×10-7）. Conclusions：LPS and IFN-γ can up-regulate IL-27 expression in CNS APCs. IL-27 expression is suppressed when CNS APCs phagocytosis of apoptotic cells.