Establishment of one-lung ventilation induced acute lung injury rabbit model by combining right mainstem bronchial intubation with parasternal fenestration

Abstract:

Objective：To build one-lung ventilation（OLV) induced acute lung injury（ALI） rabbit model by combining right mainstem bronchial intubation with parasternal fenestration. Methods：Totally 24 healthy Japanese white rabbits were randomized into 4 groups （n=6）：sham operated group（S group），two-lung ventilation（TLV） group（T group），TLV with parasternal fenestration group（TＰF group） and one-lung ventilation group（O group）. Lung injury was judged by polymorphonuclear leukocyte（PMN） count in bron－choalveolar lavage fluid（BALF），myeloperoxidase（MPO） content in pulmonary tissues，lung wet/dry（W/D） ratio and pulmonary histo－logical score. Results：Compared with those in S group，all indicators mentioned above were increased significantly in O group，T group and TF group（P<0.05）. No difference was observed between T group and TF group，but all indicators in the two groups were lower than those in O group（P<0.05）. Conclusions：Combining right mainstem bronchial intubation with parasternal fenestration is a simple and reliable method to build rabbit model of OLV induced ALI.