Preparation of targeted high molecular polymer microbubble encapsuling quantum dots and cell experiment study in vitro

Abstract:

Objective：To prepare a high molecular polymer microbubble MBQDs/PLGA/Her marked by quantum dots（QDs） and modified by single antibody Herceptin and to study its application in targeted experiment of human breast cancer cells MCF-7. Methods：MBQDs/PLGA/Her targeted microbubble was produced by the double emulsion method（W1/O/W2） and covalent coupling technique（EDC/NHS）. Its physi－cal properties（loading mark of QDs，cytotoxicity，connection of MBQDs/PLGA /Her to antibody，fluorescent marking performance of QDs） were determined by methods of TEM，MTT，Fourier transform infrared spectrum（FT-IR） and fluorescence microscope respectively. Results：MBQDs/PLGA/Her average diameter was （584.7±20.2） nm. TEM imaging showed that QDs was loaded in recombinant human tissue-type plasminogen activator poly lactic-glycolic acid（PLGA） particles and FT-IR spectrogram confirmed covalent coupling between Her－ceptin’s amino and PLGA’s carboxyl. Cell toxicity of MBQDs/PLGA/Her was less than that of QDs reagents（P<0.05）. Red microbubble MBQDs/PLGA/Her marked by QDs was connected round human breast cancer cells MCF-7. Conclusions：MBQDs/PLGA/Her microbubble is prepared successfully. Targeted microbubble cell toxicity is improved and QDs fluorescent marking performance is kept by loading QDs into polymer microbubble.