Effects of tumor invasion related protein-autocrine motility factor on biological property in human dermal fibroblasts

Abstract:

Objective：To observe the effects of autocrine motility factor（AMF） on the biological property（proliferation，anti-apoptosis and migration）of the cultured human dermal fibroblasts（HDF）. Methods：Cultured HDF were divided into experimental group（AMF group） and blank control group. Cellular anti-apoptosis and cell cycle were analyzed by flow cytometry. Proliferation and migration of the cells were respectively determined by MTT assay and Transwell chamber. Results：Abilities of proliferation（t=9.13，P<0.001），an－ti-apoptosis（t=30.94，P<0.001） and migration（t=5.35，P=0.003） were increased significantly in experimental group compared with those in blank control group. Conclusions：AMF could induce abilities of proliferation，anti-apoptosis and migration of HDF，suggest－ing that AMF is associated with keloid in characteristics of oncology.