Radioprotective effects of fructose on irradiation induced apoptosis in AHH-1 cells

Abstract:

Objective：To investigate radioprotective effects of fructose on irradiated lymphocytoblast AHH-1 and to discover the possi－ble mechanisms. Methods：Lymphocytoblasts were pretreated for 12 h before radiation with different concentrations of fructose and then were exposed to 4 Gy 60Coγ ray radiation to induce cell apoptosis. Cell survival rate was counted by MTS assay and cell apoptosis rate was detected by Annexin V/PI. Changes in mRNA expressions of Caspase-9，Bax，Bcl-2 were investigated by RT-PCR and changes in protein expressions of Caspase-9，Bax，Bcl-2 were analyzed by Western blot. Results：Irradiation increased cell death and cell survival rate was 78% after irradiation，significantly lower compared with that before irradiation（P<0.05）. If fructose was added before irradiation，cell survival rate was increased in a concentration dependent manner after irradiation（P<0.05）. mRNA and proteins expressions of Caspase-9 and Bax were significantly decreased and mRNA expressions of Bcl-2 were increased；apotososis related indexes were improved. Conclusions：Fructose exerts radioprotective effects on irradiation induced apoptosis in AHH-1 cells and its inhibition on cell apoptosis is in a concentration dependent manner.