Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography

Abstract:

Objective：To establish reserved-phase high performance liquid chromatography（HPLC） method for the determination of resveratrol content in root of polygonum cuspidatum. Methods：HPLC was carried out to detect resveratrol content in root of polygonum cuspidatum. Chromatographic conditions：mobile phase consisting of acetonitrile-water（30∶70） at flow rate of 1.0 ml/min；detection wave length of 306 nm；column temperature at room temperature；sample size of 10 μl. Results：Calibration curves were linear within the range of 0.3~9.6 μg/ml. Conclusions：The method is simple，accurate，reproducible，precise and can be used in the determination of resveratrol content in polygonum cuspidatum extract.