Experimental study on efficacy of bone marrow mesenchymal stem cell modified by plasmid pIRESneo-EGFP-BDNF in treatment of rats with Parkinson’s disease

Abstract:

Objective：To investigate efficacy of bone marrow mesenchymal stem cells（MSCs） modified by plasmid pIRESneo-EGFP-BDNF in treatment of rats with Parkinson’s disease（PD）. Methods：pIRESneo-EGFP-BDNF plasmid was established by reconstructing pEGFP（N1）-BDNF plasmid and bone marrow MSCs was transfected by electroproation. PD rat models were set up by 6-OHDA and then divided into four groups randonly：sham group，PD group，MSCs group and brain-derived neurotrophic factor（BDNF） group. Rotating behaviors of rats were induced by intraperitoneal injection of Apomorphine at 2，4，8 weeks after bone marrow MSCs transplantation or MSCs modification of plasmid pIRESneo-EGFP-BDNF through cerebral lateral ventricle. BDNF protein and tyrosine hydroxylase（TH） protein in mesencephalic nigra were measured by Western blot. Results：Plasmids pIRESneo-EGFP-BDNF were constructed successfully，which was identified by double digestion. Rotation numbers were significantly decreased in MSCs group and BDNF group than in PD group at 2，4，8 weeks after the transplantation（P<0.05），with more evident improvement in BDNF group than in MSCs group（P<0.05）. Levels of BDNF protein and TH protein in nigra at 8 weeks after PD model being intervened by cell transplantation were increased significantly in BDNF group than in MSCs group and PD group. Conclusions：Transplantation of pIRESneo-EGFP-BDNF modified MSCs through cerebral lateral ventricle can improve PD rats’ behavior and increase BDNF and TH protein levels.