Effects of NFBD1 gene silencing on apoptosis in nasopharyngeal carcinoma cells

Abstract:

Objective：To investigate the effects of nuclear factor with BRCT domains protein 1（NFBD1）gene silencing on apoptosis in nasopharyngeal carcinoma（NPC） cells. Methods：Retroviruses expressing NFBD1 shRNA were applied to infect NPC cell line HNE-1. Real-time PCR and Western bolt were used to confirm the inhibitory effects of retroviruses against NFBD1. Flow cytometry（FCM），TUNEL and Hoechest33342 staining methods were used to tested cell apoptosis. Results：NFBD1 shRNA retrovirus can inhibit mRNA and protein expressions of NFBD1 efficiently and specifically. According to the results of Hoechst33342 staining，nucleus exhibiting pycnosis and marginalization were increased significantly in experimental group than in control group（ χ2=5.367，P=0.021）. Accord－ing to the results of TUNEL staining，apoptotic cells presenting fluorescence were increased significantly in experimental group than in control group. According to the results of FCM，cell apoptosis was significantly increased in experimental group than in control group（ χ2=326.86，P＜0.001）. Conclusions：NFBD1 gene silencing in nasopharyngeal carcinoma cell line HNE-1 can effectively promote the HNE-1 apoptosis，which provide new ideas for treatment of NPC.