Association of single nucleotide polymorphism of three sites of klotho gene with left ventricle hypertrophy in elderly hypertension patients

Abstract:

Objective：To investigate the association of single nucleotide polymorphisms（SNPs） of three sites of klotho（KL） gene with left ventricle hypertrophy（LVH）in the elderly essential hypertension（EH） patients. Methods：Eligible 362 elderly patients with EH were recruited. Genomic DNA of leukocytes was extracted from venous blood and the single allele specific prime PCR technique was used to detect allele genetypes of three SNP sites of KL gene. Left ventricular mass including left ventricular end-diastolic internal dimension（LVED），left ventricular posterior wall thickness（ＬVPWT） and interventricular septal thickness（IVST） were determined by color echocardiogram simultaneously. Height and weight were also detected. Results：Frequency of allele GG genetype of KL G-395A SNP was lower in EH with LVH subgroup than in EH without LVH subgroup（P＜0.05）. According to sex stratification analysis，fre－quency of allele GG genetype was obviously lower in female EH patients with LVH than in female EH patients without LVH（P＜0.05）. Frequency of allele AA genetype of KL G-395A SNP was higher in EH with LVH subgroup than in EH without LVH subgroup（P＜0.05），but it was not significantly different between males and females. There was no difference in frequencies of allele CC，CS and SS genetypes of KL C-370S SNP and allele FF，FV and VV genetypes of KＬ F352V SNP between EH with LVH subgroup and EH without LVH subgroup（P >0.05）. Conclusions：G-395A SNP of KＬ gene is associated with LVH in elderly EH patients and female EH patients carrying GG allelotype of G-395A SNP are less likely to suffer from LVH.