Expressions of methyl-CpG-binding domain protein 2 in gastric cancer and their RNA interferences

Abstract:

Objective：To investigate the expressions of methyl-CpG-binding domain protein 2（MBD2） and their RNA interfer－ences（RNAi） in gastric cancer. Methods：Immunohistochemistry was used to detect MBD2 expressions in tissues of 40 gastric can－cer cases and matched non-cancerous tissues. Immunofluorescence was used to detect the MBD2 distribution in gastric cancer cell（SGC-7901）. Three siRNAs（MBD2-siRNA-1，MBD2-siRNA-2，MBD2-siRNA-3） targeting MBD2 were designed. LipofectamineTM2000 was used to transfect MBD2-siRNAs into SGC-7901. RT-PCR and Western blot were used to evaluate the efficiency of MBD2 inhibition. MTT assay was carried out to detect the growth rate of SGC-7901. Results：Expressions of MBD2 were higher in gastric cancer tissues than in matched gastric mucosa tissues（χ2=6.646，P=0.010）. MBD2 expressions were much higher in gastric cancer tissues with low histological grade and H.pylori infection（χ2=9.730，8.700；P=0.01，0.04）. MBD2 protein was distributed in nucleus of gastric cancer cell. Three MBD2-siRNAs all could be transfected into SGC-7901 perfectly and MBD2-siRNA-2 could inhibit the MBD2 mRNA and protein expressions best. At last，96 h after the MBD2-siRNA-2’s transfection，growth rate of SGC-7901 was inhibited. Conclusions：MBD2 protein expresses higher in gastric cancer tissues. MBD2 inhibition could suppress the proliferation of gastric cancer cell.