Association between classⅠβ-tubulin gene mutation and drug resistance of docetaxel in the treatment of advanced non-small cell lung cancer

Abstract:

Objective：To investigate the association between the mutation of the encoding gene of class Ⅰ?茁-tubulin （TUBB） and the drug resistance of docetaxel in the treatment of non-small cell lung cancer（NSCLC）. Methods：Polymerase chain reaction（PCR） and nucleotide sequencing were used to test whether mutation existed in 90 advanced NSCLC patients. Results：A mutation at the fourth ex－on of TUBB encoding sequence which encoding the 147th amino acid was found in 36 patients，but it was not significantly associated with the chemotherapy effect of decetaxel treatment for NSCLC. Conclusions：Mutation at the fourth exon of TUBB gene is not asso－ciated with the resistance of decetaxel treatment for NSCLC patients. Further investigation should be done to verify whether the struc－ture and function of tubulin would be affected by this mutation.