Differences of CD4+ CD25+ regulatory T cells between food allergy mice and normal mice

Abstract:

Objective：To compare the quantities and functions of CD4+CD25+ regulatory T（Treg）cells between food allergy mice model and normal mice model. Methods：SPF Balb/c female mice of 4-6 weeks on egg-free diet were randomly divided into ovalbumin（OVA） group（n=8） and normal control group（n=8）. Quantitative changes of CD4+CD25+ Treg cells in the spleen mononuclear cell suspension were analyzed by flow cytometry. Levels of serum interleukin-10（IL-10） and transforming growth factor-β1（TGF-β1） in OVA mice and normal control mice were detected by enzyme-linked immunosorbent assay（ELISA）. Results：Percentage of CD4+CD25+ Treg cells in OVA group was lower than that in normal control group（P<0.05），and percentage of forkhead transcription factor3+（Foxp3+）CD4+CD25+ Treg cells in OVA group was significantly lower in OVA group than in normal control group（P＜0.01）. Levels of IL-10 and TGF-β1 were lower in OVA group than in normal control group（P＜0.05）（P＜0.01）. Conclusions：There are differences in quantities and functions of Treg cells between food allergy and normal mice.