Effects of interleukin-10 modification on in vitro immunosuppression of bone marrow mesenchymal stem cells

Abstract:

Objective：To study the in vitro immunosuppression of interleukin-10（IL-10） modified bone marrow mesenchymal stem cells（BMMSCsIL-10）. Methods：IL-10 was cloned by RT-PCR and was used to construct pLNCX-IL-10. The constructed retrovirus was transfected into rat BMMSCs. T lymphocyte subsets in vitro influenced by BMMSCsIL-10 were detected by flow cytometry. Effects of BMMSCsIL-10 and its culture supernatant on in vitro mixed heterologous T lymphocyte proliferation were detected by CCK-8. Results：BMMSCsIL-10 significantly reduced CD4+ T and lowered the ratio of CD4+/CD8+，more intense than BMMSCs（P=0.000）. BMMSCsIL-10 and its culture supernatant significantly inhibited T lymphocyte proliferation，more intense than BMMSCs（P=0.000）. Conclusions：IL-10 modification can enhance the in vitro immunosuppressive effect of BMMSCs.