Expression and forensic significance of interleukin-4，interleukin-13 and immunoglobulin E in guinea pigs died of anaphylactic shock

Abstract:

Objective：To establish animal model of guinea pig anaphylactic shock，to explore the expression of interleukin-4（IL-4），interleukin-13（IL-13） and immunoglobulin E（IgE） in guinea pig serum and to provide scientific and objective evidences for clinical and medicolegal diagnosis of anaphylactic shock as well. Methods：Totally 30 guinea pigs were died of anaphylactic shock were divided into experiment group（n=20） and control group（n=10）. ELISA method was used to detect IL-4、IL-13 and IgE by extracting serum of guinea pigs in experiment group and control group. Results：Serum IL-4 level in experimental group was higher than that in control group，P=0.000；serum IL-13 level in experimental group was higher than that in control group，P=0.000；serum IgE level in experi－mental group was higher than that in control group，P=0.000. Conclusions：Serum levels of IL-4，IL-13 and IgE in experiment group are obviously higher those in control group，which provides evidences for anaphylactic shock forensic identification and clinical diag－nosis.