Measuring multiple drug resistance related gene expression in the mononuclear cells of periphery blood in child malignant tumor

Abstract:

Objective：To establish the fluorescence quantitative RT-PCR method to measure multiple drug resistance（MDR） related genes in patient’s periphery blood and to explore the feasibility of tumor chemotherapy instruction by dynamically detecting these genes in blood of patients with tumor. Methods：Taq-Man probe was applied to establish the fluorescence quantitative RT-PCR method to measure MDR related genes. Periphery blood was collected from 262 patients with tumor（including lymphoma，neuroblsatoma，yolk sac tumor，etc.） and 50 healthy persons were taken as normal controls. Mononuclear cells were isolated from these samples，then RNAs were purified and expression of MDR related genes（MDR1，MRP1 and LRP） was detected. Relationship between gene expression and tumor chemotherapy was analyzed. Results：Fluorescence quantitative RT-PCR method measuring MDR related genes was established successfully based on Taq-Man probe. Compared with that in normal control group，expression of MDR related genes（MDR1，MRP1 and LRP） was increased in mononuclear cells of periphery blood of patients with tumor. For one tumor patient，expression of MDR relat－ed genes（MDR1，MRP1 and LRP） was higher in mononuclear cells of post-chemotherapy than in those of pre-chemotherapy. Expression of MDR1，MRP1 and LRP was closely related with tumor chemotherapy scheme of patients. Conclusions：Expression of MDR related genes（MDR1，MRP1 and LRP） in periphery blood of tumor patients is dynamically measured by fluorescence quantitative RT-PCR method，which could instruct the gene related substrate chem-otherapeutic drug.