Differential expression of microRNAs in human colorectal cancer tissues

Abstract:

Objective：To identify the differentially expressed microRNAs（miRNAs） in human colorectal cancer tissues and to make further study on the function of miRNA in pathogenesis and development mechanism of colorectal cancer. Methods：Total RNA was extracted from 10 samples fresh colorectal cancer tissues and normal adjacent tissues and the differentially expressed miRNAs were detected by miRNA array. Real-time PCR was applied to verify the reliability of miRNA array results. Results：miRNA array results showed that 32 miRNAs were up-regulated more than 2.3 folds and 10 miRNAs were down-regulated more than 2 folds in colorectal cancer tissues compared with those in normal adjacent colorectal tissues. And the real-time PCR results were in accordance with the miRNA array results. Conclusion：The miRNA differential expression profile of colorectal cancer is obtained，which might relate with the pathogenesis and development of colorectal cancer.