Experiment research of curcumin induced autophagy in human medulloblastoma Daoy cell

Abstract:

Objective：To investigate the mechanism of curcumin-induced autophagy in human medulloblastoma Daoy cells. Methods：Cell viability was tested by MTT assay；expression of autophagic markers Beclin1 and LC3 was assessed by fluorescence confocal mi－croscopy，RT-PCR and Western blot；expression of PI3K，Akt，p-Akt，mTOR and p-mTOR in PI3K/Akt/mTOR signal pathway was checked by RT-PCR and Western blot. Results：Curcumin inhibited the proliferation of Daoy cells in a concentration and time depen－dent manner. Immunofluorescence showed that the intensity of Beclin1 and LC3 increased after curcumin treatment. The mRNA and protein level of Beclin1 and LC3 was increased significantly in curcumin treated group than in control group（mRNA：F=1 360.962，42.366，P=0.000，0.000；protein：F=32.723，11.847，P=0.001，0.008）. While the expression of PI3K，p-Akt and p-mTOR in curcumin treated group were lower than that in control group（F=329.662，80.638，183.536，P=0.000，0.000，0.000）. PI3K/Akt/mTOR pathway ago－nist insulin could not reverse the reduction of PI3K，p-Akt and p-mTOR induced by curcumin. Conclusion：Curcumin could inhibit the growth of human medulloblastoma Daoy cells via autophagy.