Study on methylation of ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 before and after nasopharyngeal carcinoma canceration

Abstract:

Objective：to study ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 DNA methylation feature while nasopharyngeal epithe－lium canceration. Methods：Totally 12 patients with nasopharyngeal carcinoma and 10 patients with nasopharyngitis were chose as the research objects. Nasopharyngeal tissue DNA was extracted and was used to specifically identify 5-methyl cytosine antibody immune precipitation DNA. Real-time fluorescent quantitative PCR was used to detect the contents of five genes respectively. Non 5-methyl cytosine antibody（non MeDIP-qPCR experiment） and nonspecific antibody IgG immune precipitation were set up as dual controls. Results：Regardless of the patients with nasopharyngeal carcinoma or nasopharyngitis，methylation was not detected in five genes when using nonspecific antibody IgG experiment. Non MeDIP-qPCR experiment showed that there was no difference in fluorescence quan－titative value of five genes in nasopharyngeal carcinoma and nasopharyngitis（P >0.05）. MeDIP-qPCR experiment showed that all five genes in nasopharyngeal carcinoma presented significant methylation. Corrected values of real-time fluorescence quantification of ADAM23，MIMT1，FAM150B，GRIN2A，LRRC45 were 3.39±2.28，6.03±3.93，3.68±1.81，7.12±3.17，3.10±1.94 in nasopharyn－geal carcinoma and were 0.00±0.00，0.01±0.01，0.25±0.49，0.20±0.20，0.00±0.00 in nasopharyngitis（all P < 0.01）. Conclusion：ADAM23，MIMT1，FAM150B，GRIN2A，LRRC4 gene methylation are important characteristics of nasopharyngeal carcinoma.