Transferrin modified paclitaxel and genistein co-loaded liposome for the inhibition of ovarian carcinoma cells and ovarian carcinoma bearing nude mice

Abstract:

Objective：To prepare transferrin conjugated paclitaxel（PTX） and genistein（Gen） loaded liposome and to evaluate their properties and effect on the treatment of ovarian carcinoma in vitro and in vivo. Methods：Liposomes were prepared by thin film hydra－tion method. The particle size，Zeta potential and entrapment efficiency were evaluated. Efficiency of cellular uptake and tumor sphe-roids penetration on MDR A2780 cells in vitro was evaluated. Anti-proliferation efficiency of TFLP-PTX/Gen was evaluated by MTT assay. Tumor spheroids were used to evaluate anti-tumor ability of TFLP-PTX/Gen. A2780 cells was xenografted in athymic mice to establish the animal model，which were used to evaluate the effect of anti-cancer. Results：Particle diameter of the TFLP-PTX/Gen was （115.0±9.5） nm with the Zeta potential of （-4.00±2.15） mV（Entrapment efficiency of PTX and Gen were 83.4% and 79.6% respectively）. Transferrin conjugated liposome（TFLP） uptaken by A2780 were 2.8 times higher than that of liposome（LP）. The MTT assay，the inhabitation of tumor spheroid and the inhabitation of tumor in vivo confirmed strong inhibitory effect of TFLP-PTX/Gen. Conclusions：TFLP-PTX/Gen is easy to prepare and it is a potential delivery system for the treatment of ovarian carcinoma．