Expression and clinical significances of Has-miRNA-96 in tissues of cervical cancer

Abstract:

Objective：To evaluate has-miRNA-96（miR-96） expression in cervical cancer tissues and their association with the clini－copathological features. Methods：Expression of miR-96 in 52 cases of cervical cancer tissues and 28 cases of normal tissues was examined by stem-loop real-time PCR. Correlations between expression of miR-96 and related clinicopathologic features of cervical cancer（age，pathologic pattern，histological grade，depths of interstitial infiltration，lymph node metastasis，clinical stage） were further analyzed. Results：Relative expression of miR-96 was significantly higher in cancer tissues（127.045±235.424） than in normal tis－sues（0.995±0.236）（P=0.000）. Expression of miR-96 was significantly higher in middle and low differentiated carcinoma（137.778±249.981） than in high differentiated carcinoma（75.766±147.237），with statistical differences（P=0.005）. Expression of miR-96 was significantly higher in cervical adenocarcinoma（196.213±172.519） than in squamous cell carcinomas（110.576±246.910）（P=0.004）. Expression of miR-96 at Ⅰsatge，Ⅱstage and Ⅲ-Ⅳ stage was （22.614±25.828），（122.493±78.043） and （672.902±476.169） respectively；expression at Ⅲ-Ⅳ stage was significantly higher than that at Ⅰstage and Ⅱstage（P=0.000，P=0.001） and expression at Ⅱstage was significantly higher than that at Ⅰstage（P=0.000）. Expression of miR-96 was significantly higher in those with lymphatic metastasis（142.537±104.673） than those without（19.787±26.204）（P=0.000）. Patients with invasive depth＜1/2 in－terstitial substance（27.449±49.728） showed lower level of miR-96 than those with invasive depth ≥1/2 interstitial substance（75.323±94.822）（P=0.025）. Expression of miR-96 was not correlated with age（P=0.385）. Conclusions：Aberrant expression of miR-96 sug－gests it might play a role as an oncogene in the tumorigenesis and development of cervical carcinoma and it possibly correlates with progression and prognosis of cervical cancer.