Construction and evaluation of electrospun polymethylmethacrylate nanofibers with different topographic features as biological cell scaffold

Abstract:

Objective：To produce polymethylmethacrylate（PMMA） electrospun nanofibers with different topographic features and to test their potential as scaffold after neural injury. Methods：Electrospining was used to produce nanofibers with random and aligned topographic features，PC12 cells was cultured on nanofibers and PMMA film was served as control to reveal the respective neurite growth manner on different substrate. Results：Both the random and aligned PMMA electrospun nanofibers had fine surface character and there was no difference in fiber diameter detected（t=1.079，P=0.474）. PC12 cell could successfully adhere，grow and differentiate on each kind of substrate，and on aligned nanofibers the neurite which were also aligned as the orientation of the fibers were significantly longer than those cells cultured on random fibers and film（q=35.36，P=0.006；q=29.23，P=0.009）. Conclusion：Aligned PMMA electrospun nanofibers has the potentiality as scaffold after neural injury.