Determination of the content of three nucleosides in Kangbingdu oral liquid by ultra performance liquid chromatography

Abstract:

Objective：To establish an ultra performance liquid chromatography（UPLC） method for detecting content of uridine，guanosine and adenosine in Kangbingdu oral liquid. Methods：ACQUITY UPLC BEH C１8（1.7 μm，2.1 mm×50 mm） was used and the mobile phase was methanol and water by gradient elution mode；column temperature was 30 ℃，at a flow rate of 0.5 ml/min and detection wavelength of 254 nm. Results：The linear range of uridine was 2.033-20.33 μg/ml and the regression equation was as follows：Y=80 664X-1 046（r=0.999 9）. The linear range of guanosine was 1.248-12.48 μg/ml and the regression equation was as follows：Y=55 261X-742（r=0.999 9）. The linear range of adenosine was 1.254-12.54 μg/ml and the regression equation was as follows：Y=52 155X -480（r=0.999 9）. The average recovery rates were 98.56％（RSD=1.21％），98.77％（RSD=1.09％） and 98.88％（RSD=1.22％） respectively. Conclusions：The method is accurate，rapid and reliable and can be used to determine the nucleoside compounds in Kangbingdu oral liquid.