Effects of angelica polysaccharides on bone marrow vascular endothelial growth factor in acute radiation injured mice

Abstract:

Objective：To research the protection of angelica polysaccharides（APS） on bone marrow stromal cell（ＢＭＳＣ） and vascular endothelial growth factor（ＶＥＧＦ） in radiation injured mice. Methods：BALB/c mice was irradiated by linear accelerator with one-time X ray（4.0 Gy）. Then mice were given different dosages of APS or normal saline by intraperitoneal injection. These mice were killed and the peripheral blood cells were achieved to do routine test at the 7th，14th，21st d after the injection. Adherent situation of BMSC was observed and time of 80% adherent BMSC was recorded. Cell cycle and apoptosis ratio were detected by flow cytometry. VEGF mRNA expression of BMSC was detected by RT-PCR assay. Results：Compared with those in normal group，numbers of peripheral leukocytes and platelet were decreased significantly，adherent cells of BMSC were fewer and refractivity was poorer，time of 80% ad－herent BMSC was extended significantly，percentage of G0/G1 phase and apoptosis rate were increased significantly and VEGF mRNA expression was decreased significantly in radiation group at the 7th，14th，21st d after the radiation. Conclusions：APS may promote the recovery of hematopoietic of mice bone marrow probably by enhancing expression of VEGF mRNA，then increasing proliferation ability of BMSC and reducing its apoptosis.