Analysis of SNP genetic polymorphism in CYP3A4 and CYP2D6 in Chinese Han population

Abstract:

Objective：To get the data of genetic polymorphism in the Chinese Han population through screening and detecting drug-metabolizing enzyme CYP2D6 and CYP3A4 single nucleotide polymorphism（SNP）. Methods：Eleven SNP in these 2 CYP genes were screened out. Genomic DNA was prepared from 192 blood samples obtained from individuals of Chinese Han origin. Polymor－phism was analyzed by allele-specific pr1imer extension followed by GenomeLabTM SNPstreamR analysis system. Results：All 11 ana－lyzed SNP were polymorphic（MAF>0.01）. Of these 11 SNP，7（RS28624811，RS28670611，RS9623531，RS5758589，RS3735451，RS2246709，RS2404955，RS4646440） had an allele frequency，which was different between Chinese Han population and Caucasians（P<0.01）. Conclusions：These data suggest that different ethnic groups might inherit specific genetic information that could lead to different drug metabolism rates.