Preliminary study of effect of docetaxel-loaded lipid microbubbles combined with ultrasound targeted microbubbles destruction on rabbit VX2 cervical lymph node metastatic carcinoma

Abstract:

Objective：To study the treatment effect of docetaxel-loaded lipid microbubbles（DLLM） combined with ultrasound targeted microbubbles destruction（UTMD） on rabbit VX2 cervical lymph node metastatic carcinoma. Methods：Thirty rabbit VX2 cervical lymph node metastatic carcinoma model were successfully established and were randomly divided into 6 groups（A group，B group，C group，D group，E group，F group，n=5）. A group used docetaxel，B group used DLLM，C group used docetaxel + UTMD，D group used lipid microbubbles + UTMD，E group used DLLM +UTMD and F group was control group. Volume and inhibition rate of cervical lymph node metastatic carcinoma in each group before and after the treatment were detected and compared. Expression of CD34 and microvessel density（MVD） and vascular endothelial growth factor（VEGF） was compared among different groups. Results：After the treatment，inhibition rate was higher in E group than in other groups（P<0.05） and the expression of CD34 and MVD and VEGF was significantly lower in E group than in other groups（P<0.01）. Conclusions：DLLM combined with UTMD can inhibit the growth of the cervical lymph node metastatic carcinoma from the macroscopic view.Furthermore，it can inhibit the growth of the cervical lymph node metastatic carcinoma from the molecular level.