Effect of histone deacetylase inhibitor on HBV replication

Abstract:

Objective：To investigate the effect of SIRTI inhibitor sirtinol，trichostatin A（TSA） on hepatitis B virus（HBV） replication and to discuss the role of histone deacetylase in the process of HBV replication. Methods：The tolerance degree of HepG2.2.15 cell to sirtinol and TSA was determined by MTS assay. The effects of sirtinol and TSA on the expression of HBV replicative intermediates were measured by real-time PCR and Southern blot. The effects of sirtinol and TSA on the expression of HBc，HBsAg and HBeAg were analyzed by Western blot and ELISA. Results：Sirtinol inhibited the expression of HBV replicative intermediates and HBc as well as the secretion of HBsAg and HBeAg. TSA promoted the HBV replication. Conclusion：SIRT1 inhibitor sirtinol may have anti-HBV potentiality.