High performance liquid chromatography fingerprint chromatogram and chemical pattern recognition method of Euphorbia chrysocoma Levl.etVant.

Abstract:

Objective：To study the high performance liquid chromatography（HPLC） fingerprint chromatogram and chemical pattern recognition method of Euphorbia chrysocoma Levl.etVant. Methods：Fifteen batches of Euphorbia chrysocoma Levl.etVant. samples were harvested at different time periods in different producing areas of Guizhou and were carried out to gain fingerprints by HPLC. Cluster analysis was conducted to do chemical pattern recognition of fingerprints of Euphorbia chrysocoma Levl.etVant.. Results：Fin－gerprints of 15 batches of samples demonstrated 13 characteristic peaks. Chromatographic profiles of 15 batches of samples were simi－lar；major differences resulted from intensity of chromatographic peaks. Samples were grouped into 2 types based on the harvest time. Conclusion：Components of Euphorbia chrysocoma Levl.etVant. can be well separated on HPLC chromatogram. HPLC fingerprint chromatogram has good repeatability and stability and can be used for quality control of Euphorbia chrysocoma Levl.etVant．