Determination of four alkaloids in corydalis yanhusuo by HPLC-DAD

Abstract:

Objective：To establish a high performance liquid chromatography-diode array detection（HPLC-DAD） method for the si－multaneous determination of four alkaloids in corydalis yanhusuo，and to compare the contents of four alkaloids at different production places. Methods：Angilent Eclipse XDB-C18（5 μm，4.6 mm×250 mm） was used as the analytical column. Acetonitrile-0.05% H3PO4（pH 6.0）was used as the mobile phase with gradient elution and the flow rate was 1.0 ml/min. The detection wavelengths were 270 nm for palmatine and corydaline，280 nm for tetrahydropalmatine and tetrahydroberineper respectively. Results：Palmatine，coryda－line，tetrahydropalmatine and tetrahydroberineper have a good linear relation within ranges of 1-10 μg/ml，10-100 μg/ml，5-30 μg/ml，1-10 μg/ml，respectively. Spiked recovery of high，middle and low concentration ranged from 98.2% to 101.3%. Conclusion：The method is simple，accurate and reproducible and can be used to determine the above four alkaloids．