Bioavailability of bioadhesive microspheres of silybin

Abstract:

Objective：To establish reversed phase-high performance liquid chromatography（RP-HPLC） for the determination of sily－bin in rabbit plasma and to provide a method for studying pharmacokinetics and relative bioavailability of bioadhesive microspheres of silybin. Methods：Separation was achieved using an Agilent Zorbax C18 column（250 mm×4.6 mm）.The mobile phase were：A，20 mmol/L KH2PO4 buffer，pH=3.0；B，methanol（A∶B=55∶45，V/V）. A randomized cross-over design was performed on 12 healthy rabbits. Results：Main pharmacokinetic parameters of bioadhesive microspheres and suspensions：Cmax（905.94±230.16） ng/ml and （321.32±86.65） ng/ml；T（peak）（4.70±1.02） h and （1.66±1.00） h；t1/2（4.07±0.12） h and （1.64±0.09） h；AUC0-24（11 841.35±579.58）（ng·h）/ml and （1 537.34±156.43） （ng·h）/ml，respectively. Relative bioavailability of the bioadhesive microspheres to suspensions was （770.2±370.5）%. Conclusion：Relative bioavailability of bioadhesive microspheres is 770% to suspensions of silybin and bioadhesive micro－spheres promote the absorption of silybin.