Correlation between microRNA expression profiles and VEGF of corneal neovascularization in rats

Abstract:

Objective：To study differential microRNAs（miRNAs） expression profiles in rat corneal neovascularization（CNV） using mi－croarrays，to establish the potential link between microRNAs and vascular endothelial growth factor（VEGF） signaling pathways and to investigate the function of neovascularization in corneal limbus. Methods：Corneal neovascular model in rats was constructed by corneal stitch. The LAN microarrays were selected and samples were labeled and hybridized with microarray. The results of LAN microarrays were analyzed. Results：Sixteen miRNAs were predicted to target emcee of CNV including 10 miRNAs expressing as up-regulation and 6 miRNAs expressing as down-regulation. The miRNAs expressing as up-regulation included miR-142，miR-146，miR-21，miR-223，miR-214，miR-199，miR-483，miR-322，miR-126 and miR-1224. The miRNAs expressing as down-regulation included miR-30e，miR-101，miR-184，miR-200c，miR-200b and miR-204. The miRNAs correlated significantly with VEGF included miR-142，miR-146，miR-21，miR-223，miR-199，miR-322，miR-200，miR-30e，miR-204，miR-1224. miR-142 miR-200 and miR-322 correlated most with VEGF. Conclusion：Differential expression of miR-142 miR-200 and miR-322 in the rat model of corneal neo－vascularization is closely associated with VEGF signaling pathway.