Correlation between paraoxonase1 Leu55Met gene polymorphism and chronic periodontitis with diabetes mellitus

Abstract:

Objective：To study the relationship between paraoxonase 1（PON1） Leu55Met gene polymorphisms and the risk of chronic periodontitis（CP） with diabetes. Methods：Buccal swab of 50 patients with CP and diabetes，50 patients with CP，50 patients with diabetes and 50 healthy controls were collected. DNA was isolated from buccal swabs of patients with CP and diabetes，CP，diabetes and health controls and were extracted by Chelex-100. PON1 Leu55Met gene polymorphisms were assessed by PCR amplification and digestion with restriction enzyme respectively. Results：There were statistically significant differences in the distribution of PON1 Leu55Met genotype among CP with diabetes group，CP group，diabetes group and health control group（ ?字2＝12.444，P＝0.006）. Conclu－sion：Susceptibility of CP and diabetes may associate with PON1 Leu55Met polymorphisms.