Effect of Center-Supplementing and Qi-Boosting Decoction combined siRNA on LRP expression of A549/DDP cell

Abstract:

Objective：To discuss the effect of concomitant application of small interfering RNA（siRNA） after targeted resection of lung resistance protein（LRP） and Buzhongyiqitang serum on the human lung adenocarcinoma drug-resistant cell-line A549/DDP. Methods：The siRNAs which had the same sequences of LRP gene were designed and synthesized，then the human lung adenocarcinoma drug-resistant cell-line A549/DPP was transected with transfection reagent. The experimental cells were grouped into control group，Buzhongyiqitang serum group，siRNA-processed group and Buzhongyiqitang plus siRNA-processed group. RT-PCR was applied to de－termine the mRNA expression of LRP. Western blot and immunocytochemical method were adopted to determine the protein expres－sion of LRP. Results：Protein and mRNA expression of LRP in Buzhongxiqitang serum group，siRNA group and Buzhongyiqitang plus siRNA-processed group was decreased significantly compared with that in control group（P<0.05）. Conclusion：Both Buzhongyiqitang serum and siRNA serve effectively in decreasing protein expression of LRP，and Buzhongyiqitang serum combined siRNA show syner－gistic effect.