Effects of Shenmai injection on the cytochrome P450 enzymes in cardiomyocytes

Abstract:

Objective：To investigate the influence of Shenmai injection（SMI） on mRNA expression of cytochrome P450（CYP450） in H9c2 myocardial cell. Methods：H9c2 cell was cultured by method of passage. Cell counting kit-8（ＣＣＫ－８） assay was used to detect the viability of H9c2 cells and lactate dehydrogenase（LDH） assay was used to detect the toxicity of H9c2 cells. The expression of CYP 2J3，CYP2C11，atrial natriuretic polypeptide（ANP） and brain natriuretic peptide（BNP） were measured by real-time PCR. Results：SMI could induce the expression of CYP 2J3 and CYP2C11 in H9c2 cell. In addition，SMI could induce the mRNA of ANP and BNP in a dose-dependent manner. Conclusion：SMI was widely used in the treatment of cardiovascular diseases. CYP2J3，CYP2C11，ANP and BNP mRNA expression maybe the new targets of metabolism.