Influence of HBV polymerase on virus replication with different genotypes

Abstract:

Objective：To investigate hepatitis B virus（ＨＢＶ） DNA levels with polymerase of HBV genotypes A，B，C and D. Methods：The method of restriction digestion and ligation-independent cloning（RLIC） and trans-complementation by HBV polymerase was used to express and test HBV DNA levels with four genotypes. Results：（1）HBV expressed again after HBV polymerase trans-complementa－tion. HBV DNA：A=（63.03±8.03）×105 copies/μl，B=（23.23±3.53）×105 copies/μl，C=（17.63±1.25）×105 copies/μl，D=（75.73±9.43）×105 copies/μl. （2）There were differences in HBV DNA levels among different genotype polymerases，F（4，10）=91.88，P＝0.000. （3）HBV DNA levels were higher in genotype D and A than in genotype B and C，P（A，B）=0.001，P（A，C）=0.00１，P（B，D）=0.00１，P（C，D）=0.00１. Conclusion：HBV DNA levels with polymerase of hepatitis B virus genotypes A，B，C and D were different；the mechanism of differ－ences remains to be further studied.