Preliminary study on the stability of urate oxidase loaded in lipid nanoparticles

Abstract:

Objective：To prepare urate oxidase lipid nanoparticles by Tricine-NaOH buffer（UTLNs） and then to study the in vitro ac－tivity and stability of free UOX and UTLNs. Methods：UTLNs was prepared by reverse-phase evaporation method. The optimum tem－perature，optimum pH，thermal stability，storage stability，pH stabilities and proteolytic stabilities of free UOX and UTLNs were mea－sured. Results：The optimum temperatures for free UOX and UTLNs were both 40 ℃. The optimum pH for free UOX and UTLNs were 8.5 and 8.0，respectively. UTLNs had significantly better stability than free UOX in vitro. Conclusion：UTLNs not only improved the activity but also significantly enhanced the stability of free UOX in vitro.