Dose-dense administration of combinative paclitaxel and cisplatin might suppress the repopulation of ovarian cancer cell SKOV3

Abstract:

Objective：To compare the impact of different dosage regimens of cisplatin（CDDP） or/and paclitaxel（PTX） on the repopu－lation of ovarian cancer cells. Methods：（1）SKOV3 cells were treated with CDDP，PTX or CDDP combined with PTX respective－ly，each arm treated with fractional dose regimen（the total dosage was dividedly administration on d1 and d8） or single dose regimen（the total dosage was administration on d1）；（2）The apoptosis and repopulation of SKOV3 cells were observed under microscope；（3）The cell survival on d7 and d21 in each group was detected by CCK-8 method. Results：（1）In CDDP groups，no significant dif－ference was found between the two regimens（P=0.970）；（2）In PTX groups，the repopulation of SKOV3 cells in fractional dose regi－men was significantly inhibited compared with that of single dose regimen（P=0.000）；（3）Fractional schedule significantly inhibited the repopulation of SKOV3 cells in CDDP+PTX groups（P=0.000），and such suppressive efficiency was even more evident in CDDP+PTX group than in PTX monotherapy group（P=0.000）. Conclusion：CDDP+PTX with fractional dose regimen might prevent resistance or recurrence of ovarian cancer cells through significantly inhibiting the repopulation of ovarian cancer cells.