Expression and localization of the spermatogenesis-related gene Znf230 in mouse testis and spermatozoa

Abstract:

Objective：To detect the expression and location of Znf230 in mouse testis and spermatozoa by Znf230 polyclonal anti－serum. Methods：Expression and localization of Znf230 in mouse testis at different developmental stages and epididymal spermato－zoa were determined by RT-PCR，immunoblotting，immunohistochemistry and immunoluorescence. Results：Znf230 remained relatively stable and was expressed throughout postnatal development of the mouse testis. It was primarily expressed in the nuclei of spermato－gonia（from postnatal d 6 to postnatal d 12） and subsequently in the acrosome system and the entire tail of round spermatids，elongated spermatids and spermatozoa（from postnatal d 18 to mature sperm）. Conclusion：Znf230 may play an important role in mouse spermatogenesis.