Expression and significance of transforming growth factor-β1 in the apoptosis of rat spermatogenic cells induced by cytoxan

Abstract:

Objective：To preliminarily assess the expression and significance of transforming growth factor-β1（TGF-β1） in the apop－tosis of rat spermatogenic cells induced by cytoxan. Methods：Cytoxan was applied for male Wistar rats aged 9 d. After 24 h，4 weeks and 8 weeks，apoptosis of spermatogonia was counted severally by TUNEL and TGF-β1 was detected severally by RT-PCR in testes of 10 rats in experimental group and control group. Results：（1）There was no difference in the apoptosis of spermatogenic cell and TGF-β1 expression between control group and experimental group at 24 h after the treatment at 24 h after the treatment. （Fbetween groups=1.25，P=0.924）（Fbetween groups=1.59，P=0.339）（2）Apoptosis of spermatogenic cell and TGF-β1 expression were significantly increased in experimental groups than in control group（Fbetween groups=126.73，P=0.005）（Fbetween groups=32.86，P=0.007）. Conclusion：Cytoxan not only increases apoptosis of spermatogenic cell in testes，but also results in elevated expression of TGF-β1 of testes in rat，indicating that increased expression of TGF-β1 in testes will lead to increased apoptosis of spermatogenic cell in testes.