Effect of human adipose-derived stem cells on function of human fibroblasts of different ages in Transwell system

Abstract:

Objective：To investigate the effect of adipose-derived stem cells（ADSCs） on young and elderly derived fibroblasts in Tran－swell system. Methods：ADSCs were isolated from human adipose tissues and were cultured. Fibroblasts derived from young people and old people were isolated from human skin tissue and were cultured. ADSCs and fibroblasts were co-cultured in Transwell chamber. Fibroblasts proliferation was evaluated by cell proliferation and cytotoxicity assay kit. Expression of fibroblasts collagen typeⅠand matrix metalloproteinase-1（MMP-1） and β-galactosidase mRNA were evaluated by RT-PCR. Results：（1）The proliferation of youth experimental group and elderly experimental group were obviously higher than that of young control group and elderly control group（P=0.000），and the proliferation of youth experimental group is higher than that of elderly experimental group（P=0.000）. （2）The ex－pression of collagen typeⅠand β-galactosidase mRNA of youth experimental group and elderly experimental group was obviously higher than that of youth control group and elderly control group（P=0.000），and the expression of collagen typeⅠand β-galactosidase mRNA of youth experimental group was higher than that of elderly experimental group（P=0.002，P=0.000）. （3）The expression of MMP-1 mRNA of youth experimental group and elderly experimental group was obviously lower than that of youth control group and elderly control group（P=0.000），but the expression of MMP-1 mRNA of youth experimental group was lower than that of elderly experimental group（P=0.055）；the results may relate with the proliferation of fibroblasts and decreased production of MMP-1 in the elderly and the young after the co-culture. Conclusion：Fibroblasts cultured with ADSCs can promote fibroblasts proliferation and collagen secretion.